mouse control igg Search Results


biotinylated igg anti mouse  (Vector Laboratories)
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Vector Laboratories biotinylated igg anti mouse
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mouse igg1  (InvivoGen)
94
InvivoGen mouse igg1
Mouse Igg1, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hrp conjugated goat anti mouse igg2a proteintech group cat  (Proteintech)
96
Proteintech hrp conjugated goat anti mouse igg2a proteintech group cat
Figure 3. Titration of rtN1- and rtN2-spe- cific serum <t>IgG</t> in ELISA The sera samples collected 2 weeks after (A and D) prime immunization, (B and E) first booster, and (C and F) second booster were titrated for (A–C) rtN1 and (D–F) rtN2. Each dot represents the serum antibody titer from one mouse (n = 5 per group). Bars represent the mean values of serum antibody titers. The dash line represents the limit of detection, corresponding to the initial serum dilution. The positive wells were determined as OD values above mean +2 3 SD of the OD value of the initial pre-immune sera dilution. Statistical signifi- cance was analyzed by t test for (B), (C), (E), and (F). *p < 0.05, **p < 0.01, and ****p < 0.001. All experiments were repeated twice using the same serum samples and showed similar results.
Hrp Conjugated Goat Anti Mouse Igg2a Proteintech Group Cat, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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igg control  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc igg control
Figure 3. Titration of rtN1- and rtN2-spe- cific serum <t>IgG</t> in ELISA The sera samples collected 2 weeks after (A and D) prime immunization, (B and E) first booster, and (C and F) second booster were titrated for (A–C) rtN1 and (D–F) rtN2. Each dot represents the serum antibody titer from one mouse (n = 5 per group). Bars represent the mean values of serum antibody titers. The dash line represents the limit of detection, corresponding to the initial serum dilution. The positive wells were determined as OD values above mean +2 3 SD of the OD value of the initial pre-immune sera dilution. Statistical signifi- cance was analyzed by t test for (B), (C), (E), and (F). *p < 0.05, **p < 0.01, and ****p < 0.001. All experiments were repeated twice using the same serum samples and showed similar results.
Igg Control, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal antibody against sdf 1  (R&D Systems)
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R&D Systems mouse monoclonal antibody against sdf 1
Figure 3. Titration of rtN1- and rtN2-spe- cific serum <t>IgG</t> in ELISA The sera samples collected 2 weeks after (A and D) prime immunization, (B and E) first booster, and (C and F) second booster were titrated for (A–C) rtN1 and (D–F) rtN2. Each dot represents the serum antibody titer from one mouse (n = 5 per group). Bars represent the mean values of serum antibody titers. The dash line represents the limit of detection, corresponding to the initial serum dilution. The positive wells were determined as OD values above mean +2 3 SD of the OD value of the initial pre-immune sera dilution. Statistical signifi- cance was analyzed by t test for (B), (C), (E), and (F). *p < 0.05, **p < 0.01, and ****p < 0.001. All experiments were repeated twice using the same serum samples and showed similar results.
Mouse Monoclonal Antibody Against Sdf 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mouse igg  (Vector Laboratories)
99
Vector Laboratories anti mouse igg
Figure 3. Titration of rtN1- and rtN2-spe- cific serum <t>IgG</t> in ELISA The sera samples collected 2 weeks after (A and D) prime immunization, (B and E) first booster, and (C and F) second booster were titrated for (A–C) rtN1 and (D–F) rtN2. Each dot represents the serum antibody titer from one mouse (n = 5 per group). Bars represent the mean values of serum antibody titers. The dash line represents the limit of detection, corresponding to the initial serum dilution. The positive wells were determined as OD values above mean +2 3 SD of the OD value of the initial pre-immune sera dilution. Statistical signifi- cance was analyzed by t test for (B), (C), (E), and (F). *p < 0.05, **p < 0.01, and ****p < 0.001. All experiments were repeated twice using the same serum samples and showed similar results.
Anti Mouse Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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control migg1  (R&D Systems)
96
R&D Systems control migg1
Figure 3. Titration of rtN1- and rtN2-spe- cific serum <t>IgG</t> in ELISA The sera samples collected 2 weeks after (A and D) prime immunization, (B and E) first booster, and (C and F) second booster were titrated for (A–C) rtN1 and (D–F) rtN2. Each dot represents the serum antibody titer from one mouse (n = 5 per group). Bars represent the mean values of serum antibody titers. The dash line represents the limit of detection, corresponding to the initial serum dilution. The positive wells were determined as OD values above mean +2 3 SD of the OD value of the initial pre-immune sera dilution. Statistical signifi- cance was analyzed by t test for (B), (C), (E), and (F). *p < 0.05, **p < 0.01, and ****p < 0.001. All experiments were repeated twice using the same serum samples and showed similar results.
Control Migg1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ca mouse igg1  (Miltenyi Biotec)
96
Miltenyi Biotec ca mouse igg1
Figure 3. Titration of rtN1- and rtN2-spe- cific serum <t>IgG</t> in ELISA The sera samples collected 2 weeks after (A and D) prime immunization, (B and E) first booster, and (C and F) second booster were titrated for (A–C) rtN1 and (D–F) rtN2. Each dot represents the serum antibody titer from one mouse (n = 5 per group). Bars represent the mean values of serum antibody titers. The dash line represents the limit of detection, corresponding to the initial serum dilution. The positive wells were determined as OD values above mean +2 3 SD of the OD value of the initial pre-immune sera dilution. Statistical signifi- cance was analyzed by t test for (B), (C), (E), and (F). *p < 0.05, **p < 0.01, and ****p < 0.001. All experiments were repeated twice using the same serum samples and showed similar results.
Ca Mouse Igg1, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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isotype control igg1  (R&D Systems)
96
R&D Systems isotype control igg1
Figure 3. Titration of rtN1- and rtN2-spe- cific serum <t>IgG</t> in ELISA The sera samples collected 2 weeks after (A and D) prime immunization, (B and E) first booster, and (C and F) second booster were titrated for (A–C) rtN1 and (D–F) rtN2. Each dot represents the serum antibody titer from one mouse (n = 5 per group). Bars represent the mean values of serum antibody titers. The dash line represents the limit of detection, corresponding to the initial serum dilution. The positive wells were determined as OD values above mean +2 3 SD of the OD value of the initial pre-immune sera dilution. Statistical signifi- cance was analyzed by t test for (B), (C), (E), and (F). *p < 0.05, **p < 0.01, and ****p < 0.001. All experiments were repeated twice using the same serum samples and showed similar results.
Isotype Control Igg1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse igg2a isotype control antibody  (R&D Systems)
95
R&D Systems mouse igg2a isotype control antibody
Figure 3. Titration of rtN1- and rtN2-spe- cific serum <t>IgG</t> in ELISA The sera samples collected 2 weeks after (A and D) prime immunization, (B and E) first booster, and (C and F) second booster were titrated for (A–C) rtN1 and (D–F) rtN2. Each dot represents the serum antibody titer from one mouse (n = 5 per group). Bars represent the mean values of serum antibody titers. The dash line represents the limit of detection, corresponding to the initial serum dilution. The positive wells were determined as OD values above mean +2 3 SD of the OD value of the initial pre-immune sera dilution. Statistical signifi- cance was analyzed by t test for (B), (C), (E), and (F). *p < 0.05, **p < 0.01, and ****p < 0.001. All experiments were repeated twice using the same serum samples and showed similar results.
Mouse Igg2a Isotype Control Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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isotype control mouse igg1  (Miltenyi Biotec)
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Miltenyi Biotec isotype control mouse igg1
Figure 3. Titration of rtN1- and rtN2-spe- cific serum <t>IgG</t> in ELISA The sera samples collected 2 weeks after (A and D) prime immunization, (B and E) first booster, and (C and F) second booster were titrated for (A–C) rtN1 and (D–F) rtN2. Each dot represents the serum antibody titer from one mouse (n = 5 per group). Bars represent the mean values of serum antibody titers. The dash line represents the limit of detection, corresponding to the initial serum dilution. The positive wells were determined as OD values above mean +2 3 SD of the OD value of the initial pre-immune sera dilution. Statistical signifi- cance was analyzed by t test for (B), (C), (E), and (F). *p < 0.05, **p < 0.01, and ****p < 0.001. All experiments were repeated twice using the same serum samples and showed similar results.
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mouse igg2a isotype control pe  (R&D Systems)
94
R&D Systems mouse igg2a isotype control pe
Figure 3. Titration of rtN1- and rtN2-spe- cific serum <t>IgG</t> in ELISA The sera samples collected 2 weeks after (A and D) prime immunization, (B and E) first booster, and (C and F) second booster were titrated for (A–C) rtN1 and (D–F) rtN2. Each dot represents the serum antibody titer from one mouse (n = 5 per group). Bars represent the mean values of serum antibody titers. The dash line represents the limit of detection, corresponding to the initial serum dilution. The positive wells were determined as OD values above mean +2 3 SD of the OD value of the initial pre-immune sera dilution. Statistical signifi- cance was analyzed by t test for (B), (C), (E), and (F). *p < 0.05, **p < 0.01, and ****p < 0.001. All experiments were repeated twice using the same serum samples and showed similar results.
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Image Search Results


Figure 3. Titration of rtN1- and rtN2-spe- cific serum IgG in ELISA The sera samples collected 2 weeks after (A and D) prime immunization, (B and E) first booster, and (C and F) second booster were titrated for (A–C) rtN1 and (D–F) rtN2. Each dot represents the serum antibody titer from one mouse (n = 5 per group). Bars represent the mean values of serum antibody titers. The dash line represents the limit of detection, corresponding to the initial serum dilution. The positive wells were determined as OD values above mean +2 3 SD of the OD value of the initial pre-immune sera dilution. Statistical signifi- cance was analyzed by t test for (B), (C), (E), and (F). *p < 0.05, **p < 0.01, and ****p < 0.001. All experiments were repeated twice using the same serum samples and showed similar results.

Journal: Cell reports

Article Title: Boost immunizations with NA-derived peptide conjugates achieve induction of NA inhibition antibodies and heterologous influenza protections.

doi: 10.1016/j.celrep.2023.112766

Figure Lengend Snippet: Figure 3. Titration of rtN1- and rtN2-spe- cific serum IgG in ELISA The sera samples collected 2 weeks after (A and D) prime immunization, (B and E) first booster, and (C and F) second booster were titrated for (A–C) rtN1 and (D–F) rtN2. Each dot represents the serum antibody titer from one mouse (n = 5 per group). Bars represent the mean values of serum antibody titers. The dash line represents the limit of detection, corresponding to the initial serum dilution. The positive wells were determined as OD values above mean +2 3 SD of the OD value of the initial pre-immune sera dilution. Statistical signifi- cance was analyzed by t test for (B), (C), (E), and (F). *p < 0.05, **p < 0.01, and ****p < 0.001. All experiments were repeated twice using the same serum samples and showed similar results.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-63His tag Sino Biological Cat#105327-MM02T-H Rabbit polyclonal anti-N1 Sino Biological Cat#11058-R001 Rabbit polyclonal anti-N2 Sino Biological Cat#40017-RP01 HRP-conjugated goat anti-human IgG Proteintech Group Cat#SA00001-17 HRP-conjugated goat anti-mouse IgG Proteintech Group Cat#SA00001-1 HRP-conjugated goat anti-mouse IgG1 Proteintech Group Cat#SA00012-1 HRP-conjugated goat anti-mouse IgG2a Proteintech Group Cat#SA00012-2 HRP-conjugated goat anti-mouse IgG3 Proteintech Group Cat#SA00012-5 HRP-conjugated goat anti-mouse IgM Proteintech Group Cat#SA00012-6 HRP-conjugated peanut agglutinin Sigma-Aldrich Cat#L7759 Bacterial and virus strains E.coli DH5a competent cells Lab stock N/A E.coli DH10Bac competent cells Lab stock N/A A/Hong Kong/8/1968 (H3N2) ATCC Cat#VR544TM A/New Jersey/8/1976 (H1N1) ATCC Cat#VR897TM A/Puerto Rico/8/1934 (H1N1) Haiyan Chang, Hunan Normal University N/A A/reassortant/NYMC X179A (H1N1) Haiyan Chang, Hunan Normal University N/A A/Guizhou/54/1989 (GZ89, H3N2) Haiyan Chang, Hunan Normal University N/A A/Puerto Rico/8/1934 (H1N1) Haiyan Chang, Hunan Normal University N/A Biological samples Human convalescent sera samples Yao-Qing Chen’s laboratory stock N/A Chemicals, peptides, and recombinant proteins N1P1 Top-peptide N/A N1P2 Top-peptide N/A N1P3 Top-peptide N/A N1P4 Top-peptide N/A N2P1 Top-peptide N/A N2P2 Top-peptide N/A N2P3 Top-peptide N/A N2P4 Top-peptide N/A rtN1 This paper N/A rtN2 This paper N/A KLH Solarbio Cat#K8160 Receptor destroying enzyme Denka Seiken Cat#340122 Fetuin from fetal bovine serum Sigma-Aldrich Cat#F2379 Incomplete Freund’s adjuvant Sigma-Aldrich Cat#F5506 Critical commercial assays ReadiLinkTM KLH Conjugation Kit AAT Bioquest Cat#5502 Mouse IFNg ELISPOT Kit BD Biosciences Cat#551083 Mouse IL-4 ELISPOT Kit Dakewe Cat#DKW22-2040-500 (Continued on next page) Cell Reports 42, 112766, July 25, 2023 11

Techniques: Titration, Enzyme-linked Immunosorbent Assay